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发表于 2011-10-13 18:24 · 湖北
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本帖最后由 rexivano 于 2011-10-13 18:25 编辑
nozxxx 发表于 2011-10-13 18:22 
要死了..............
恩…………
给你看看我正在做的作业
翻译
1. Introduction
Vacuoles in plant cells have numerous functions, some of
which appear to be mutually incompatible. For example,
vacuoles contain proteases with optimal activity at acid pH
[1,2], yet vacuoles may also provide an environment where
proteins can be stored and protected from acid proteases that
are present within the same organelles [3]. One possible
explanation for this apparent contradiction, the presence of
an acidic lytic environment versus an environment optimal
for protein storage and stability, is that lytic and storage
vacuoles may exist as separate organelles [4,5],or,
alternatively, that one vacuole may contain separate
compartments separated by membranes [6,7].Thenumber
of different vacuole types and their interactions with each
other appear to be developmentally regulated [4].The
precedent from other systems [8,9] would require that
distinct vacuoles be served by traffic from distinct types of
transport vesicles. In that regard, we previously postulated
that internal vesicles in multivesicular body prevacuolar
compartments may represent an internal compartment served
by transport vesicles different from those delivering cargo to
the surrounding ‘‘soup’’ of the organelle [7]. In that regard, it
is of note that recent electron microscopic studies using
Arabidopsis embryos that were prepared with high pressure
freezing/freeze substitution to prevent membrane fixation
artifacts demonstrated that internal vesicles in multivesicular
bodies contained electron dense contents [10,11].These
observations may be consistent with delivery of transport
vesicle contents to the internal vesicles, rather than formation
of the internal vesicles by simple invagination of the
organelle limiting membrane.
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